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Hycult Biotech
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Image Search Results
Journal: Biomolecules
Article Title: Endogenous Glycoprotein GPM6a Is Involved in Neurite Outgrowth in Rat Dorsal Root Ganglion Neurons
doi: 10.3390/biom13040594
Figure Lengend Snippet: GPM6a’s detection in rat DRG explants. Representative images of embryonic DRGs at 10 DIV ( a , b ) and 21 DIV ( c ). N = 2–3 independent experiments with n = 3–8 DRG explants per condition were analyzed. Images show positive immunostaining for GPM6a (GPM6a-mAb, shown in magenta), axons (neurofilament, NFh-mAb, or Thy-1-mAb, shown in green), and SCs (S100β in green). Cell nuclei are shown in cyan. Confocal images were acquired with an Olympus FV1000 confocal microscope using the following settings: ( a , b ) 20× objective, insets: 220 × 220 µm; ( c ) 20× objective, inset: 120 × 120 µm. Images from panels ( a , b ) are maximal projections of 11 z-stacks and 10 z-stacks respectively, using a step size of 1 µm in both cases. Scale bars are indicated in the figure.
Article Snippet: Monoclonal antibodies produced in-house from hybridoma cell cultures were anti-neurofilament heavy chain mouse IgG (NFh-mAb), hybridoma cell line RT97 (DSHB, Iowa City, IA, USA), and
Techniques: Immunostaining, Microscopy